Vinson’s chapter titled “Practical Approaches for Cryo-FIB Milling and Applications for Cellular Cryo-Electron Tomography” is published as part of the cryoEM. Methods in Molecular Biology book edited by Tamir Gonen and Brent Nannenga. It has a lot of useful tips and we hope it will help #democratizecryoET.
Our collaboration with the lab of Don Cleveland describing how TDP-43 and HSP70 phase separate into anisotropic, intranuclear liquid spherical annuli is now published after peer review in Science (see preprint here). We’re happy to have contributed to this exciting work. Congratulations Digvijay on capturing these phase separated compartments in the nucleus using cryo-ET!
Our collaboration with the labs of Andres Leschziner, Sam Reck-Peterson, Stefan Knapp and Andy Shiau describing the high-resolution structure of LRRK2, and testing the hypothesis of whether LRRK2 can block motors on microtubules is now published after peer review in Nature (see preprint here) Congratulations to Reika and Jan on this fantastic collaboration. Many more exciting stories to come!
Kanika’s review on the molecular architecture and transformations of B. subtilis during sporulation it out on Annual Review of Microbiology.
Our paper describing how to cryo-FIB mill cells for cryo-ET is out in Nature Protocols. Congratulations Felix, Reika, and Digvijay for the great work helping democratize cryo-ET!
Our paper showing how virus capsids “surf” on microtubule-like PhuZ filaments toward a nucleus they build when infecting bacteria is out. Bacterial Cell Biology at it’s weirdest!
Kanika’s work on bacterial engulment is posted in the bioRxiv.
Elizabeth was honored to serve as co-guest editor for Klaus Schulten’s Memorial issue, published in The Journal of Physical Chemistry B.