The Villa Lab develops tools to observe macromolecular complexes in their natural environment, the cell. Our ultimate goal is to unveil the structural dynamics of these complexes while they go about their everyday life. We combine cell biology and electron microscopy to generate data, and we use image processing and physical modeling to understand these data. Current projects include: the structural dynamics of the yeast nuclear pore complex, mechanotransduction between the nucleus and the cytoplasm, microbial cytoskeleton, and development of techniques to create new tags for correlative light and electron microscopy.