Our paper in collaboration with the Shapiro lab was posted to bioRxiv. This work deciphers the molecular grammar of the bacterial protein PopZ in forming phase-separated compartments, and uses PopZ as a synthetic tool in human cells. Vinson used cryo-ET to show that altering protein domain composition changes structural order of the compartments and affects ribosome distribution in cells.
Vinson’s chapter titled “Practical Approaches for Cryo-FIB Milling and Applications for Cellular Cryo-Electron Tomography” is published as part of the cryoEM. Methods in Molecular Biology book edited by Tamir Gonen and Brent Nannenga. It has a lot of useful tips and we hope it will help #democratizecryoET.
Our collaboration with the lab of Don Cleveland describing how TDP-43 and HSP70 phase separate into anisotropic, intranuclear liquid spherical annuli is now published after peer review in Science (see preprint here). We’re happy to have contributed to this exciting work. Congratulations Digvijay on capturing these phase separated compartments in the nucleus using cryo-ET!
Elizabeth gives her tenure talk. It has been a challenging year, but we are grateful for the opportunity to do great science in a wonderful environment.
Josh is awarded an EMBO long-term fellowship. Congratulations!
Matt has advanced to candidacy! Congratulations on the great progress in your high-risk projects!
Happy Postdoc Appreciation Week! Our postdocs are creative, independent, intelligent and hard working scientists. We are lucky to have them!
Our collaboration with the labs of Andres Leschziner, Sam Reck-Peterson, Stefan Knapp and Andy Shiau describing the high-resolution structure of LRRK2, and testing the hypothesis of whether LRRK2 can block motors on microtubules is now published after peer review in Nature (see preprint here) Congratulations to Reika and Jan on this fantastic collaboration. Many more exciting stories to come!
Our work describing the in situ architecture of a Parkinson’s disease-linked LRRK2 has now been published after peer review in Cell (see preprint here).